Department of Dermatological, Jinan City People's Hospital, Laiwu District, Jinan 271100, Shandong, PR China.
Department of Dermatological, The Second Affiliated Hospital of Shandong First Medical University, Taian 271000, Shandong, PR China.
J Nanosci Nanotechnol. 2021 May 1;21(5):3028-3034. doi: 10.1166/jnn.2021.19155.
This study's objective is to analyze the effect of epidermal growth factor (EGF) nanoparticles on the healing of diabetic skin wounds and also, simultaneously, to investigate the mechanism of EGF nanoparticles to promote healing. In this manuscript, EGF nanoparticles were prepared, and also the drug loading rate of EGF nanoparticles was measured. In the meantime, a diabetic skin wound model was prepared with the use of rats. Then, the rats were split into four groups: EGF nanogroup, EGF group, empty particle group, and control group. Additionally, the results indicate that this study was successful in preparing EGF nanoparticles with a stable performance, and the drug was released for 24 hours. The wound healing in the EGF nanoparticle group was quicker than that in the EGF group. Furthermore, the area of EGF receptor-positive cells in the wound surface of the EGF nanogroup was higher than that of the EGF group, with the results demonstrating that EGF nanoparticles upregulated the expression of EGF receptors in wound surface cells, promoted wound surface healing, and had better efficacy than EGF.
本研究旨在分析表皮生长因子（EGF）纳米粒子对糖尿病皮肤伤口愈合的影响，并同时探讨 EGF 纳米粒子促进愈合的机制。在本研究中，我们制备了 EGF 纳米粒子，并测量了 EGF 纳米粒子的载药量。同时，我们使用大鼠制备了糖尿病皮肤伤口模型。然后，将大鼠分为四组：EGF 纳米组、EGF 组、空颗粒组和对照组。此外，结果表明，本研究成功制备了性能稳定的 EGF 纳米粒子，药物可在 24 小时内释放。EGF 纳米粒子组的伤口愈合速度快于 EGF 组。此外，EGF 纳米组伤口表面 EGF 受体阳性细胞的面积高于 EGF 组，结果表明 EGF 纳米粒子上调了伤口表面细胞中 EGF 受体的表达，促进了伤口表面愈合，疗效优于 EGF。
