Saboor Soomro Rukhsana, Shah Iftikhar Ali, Saboor Abdul, Bhutto Aman Ullah B, Memon Sidra
Pathology, Ghulam Muhammad Mahar Medical College Hospital, Sukkur, PAK.
Internal Medicine, Ghulam Muhammad Mahar Medical College Hospital, Sukkur, PAK.
Cureus. 2021 Jan 25;13(1):e12909. doi: 10.7759/cureus.12909.
In low-income and high hepatitis B and C virus burden countries like Pakistan, it is important to develop cheap yet efficient strategies in diagnosing as well as treating hepatitis. The aim of this study is to assess the sensitivity and specificity of serum hepatitis B surface antigen (HbsAg) via Rapid Immunoassay Chromatographic Test (RICT) for the screening of hepatitis B, compared to the gold standard laboratory-based method.
The study was conducted in the Hepatology Clinic of Civil Hospital, Sukkur. All records of the clinic from June 1, 2018, to December 31, 2018, were accessed for identification of the records in which hepatitis B screening via RICT and then confirmatory polymerase chair reaction (PCR) by gene amplification with forward and reverse primers was done.
There were 151 samples in this study. There were 32 (94.1%) true-positive and three (5.8%) false-negative samples. There were two (2.5%) false-positive and 114 (97.4%) true-negative samples. The sensitivity of HbsAg detection via RICT for the screening of 1-1B V was 91.43%, specificity was 98.28% and the accuracy was 96.69%, compared to PCR.
The RICT method has high sensitivity and specificity. In low-income and high-hepatitis B virus-burden countries like Pakistan, it serves as a very efficient screening tool that is easy to use, cheaper in cost, and gives rapid and accurate results.
在巴基斯坦这样的低收入且乙肝和丙肝病毒负担较高的国家，制定廉价而有效的肝炎诊断和治疗策略非常重要。本研究的目的是通过快速免疫分析色谱法（RICT）评估血清乙肝表面抗原（HbsAg）用于乙肝筛查的敏感性和特异性，并与基于实验室的金标准方法进行比较。
该研究在苏库尔市民医院的肝病诊所进行。查阅了该诊所2018年6月1日至2018年12月31日的所有记录，以确定那些先通过RICT进行乙肝筛查，然后通过使用正向和反向引物进行基因扩增的验证性聚合酶链反应（PCR）的记录。
本研究中有151个样本。有32个（94.1%）真阳性和3个（5.8%）假阴性样本。有2个（2.5%）假阳性和114个（97.4%）真阴性样本。与PCR相比，通过RICT检测HbsAg用于乙肝筛查的敏感性为91.43%，特异性为98.28%，准确性为96.69%。
RICT方法具有高敏感性和特异性。在巴基斯坦这样的低收入且乙肝病毒负担较高的国家，它是一种非常有效的筛查工具，易于使用，成本较低，且能给出快速准确的结果。
