一种用于研究对细菌病原体和害虫的诱导系统抗性（ISR）的改良生物测定法。
An Improved Bioassay to Study Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests.
作者信息
Cecchini Nicolás M, Song Yi, Roychoudhry Suruchi, Greenberg Jean T, Haney Cara H
机构信息
Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Departamento de Química Biológica-Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Córdoba X5000HUA, Argentina.
Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
出版信息
Bio Protoc. 2019 May 20;9(10):e3236. doi: 10.21769/BioProtoc.3236.
The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of spp. with the reference plant . Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen pv. (formerly called strain ES4326 and the herbivore by inoculating strain WCS417 (formerly called WCS417) on plants grown in Jiffy-7 peat pellets. While most commonly used for triggered ISR on , this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores.
植物免疫系统对于植物感知并抵御细菌、真菌、害虫和病原体至关重要。诱导系统抗性（ISR）是一种在有益微生物定殖于根部时发生的系统免疫反应。一个经过充分研究的ISR模型是特定有益菌株与模式植物的关联。在此，我们描述了一种强大的、通量增加的生物测定法，通过在Jiffy-7泥炭丸中生长的植物上接种WCS417菌株（原称WCS417），来研究针对细菌病原体pv.（原称菌株ES4326）和食草动物的ISR。虽然该测定法最常用于在植物上引发ISR，但它对多种根际细菌菌株、植物物种、病原体和食草动物均有效。
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