Haney Cara H, Samuel Buck S, Bush Jenifer, Ausubel Frederick M
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Nat Plants. 2015;1(6). doi: 10.1038/nplants.2015.51. Epub 2015 May 11.
Host-associated microbiomes influence host health. However, it is unclear whether genotypic variations in host organisms influence the microbiome in ways that have adaptive consequences for the host. Here, we show that wild accessions of differ in their ability to associate with the root-associated bacterium , with consequences for plant fitness. In a screen of 196 naturally occurring accessions we identified lines that actively suppress growth under gnotobiotic conditions. We planted accessions that support disparate levels of fluorescent Pseudomonads in natural soils; 16S ribosomal RNA sequencing revealed that accession-specific differences in the microbial communities were largely limited to a subset of Pseudomonadaceae species. These accession-specific differences in growth resulted in enhanced or impaired fitness that depended on the host's ability to support growth, the specific strains present in the soil and the nature of the stress. We suggest that small host-mediated changes in a microbiome can have large effects on host health.
与宿主相关的微生物群会影响宿主健康。然而，尚不清楚宿主生物体的基因型变异是否会以对宿主具有适应性影响的方式影响微生物群。在这里，我们表明，[植物名称]的野生种质在与根际细菌[细菌名称]结合的能力上存在差异，这对植物适应性产生了影响。在对196份天然存在的[植物名称]种质进行的筛选中，我们鉴定出了在无菌条件下能积极抑制[细菌名称]生长的株系。我们在天然土壤中种植了对荧光假单胞菌支持水平不同的种质；16S核糖体RNA测序显示，微生物群落中种质特异性差异主要局限于假单胞菌科物种的一个子集。这些在[细菌名称]生长方面的种质特异性差异导致适应性增强或受损，这取决于宿主支持[细菌名称]生长的能力、土壤中存在的特定[细菌名称]菌株以及胁迫的性质。我们认为，宿主介导的微生物群的微小变化可能会对宿主健康产生重大影响。
