Pischedda Francesca, Piccoli Giovanni
CIBIO, Dulbecco Telethon Institute, University of Trento, Trento, Italy.
Bio Protoc. 2019 Jun 20;9(12):e3270. doi: 10.21769/BioProtoc.3270.
Primary neuronal culture from rodents is a key tool in neurobiology. However, the preparation of primary cultures requires precise planning, starting from animal mating. Furthermore, each preparation generates a high amount of cells that eventually go wasted. The possibility to cryopreserve primary neural cells represents a resource for studies and significantly reduces the sacrifice of animals. Here we describe that Neurostore buffer supports the cryopreservation of primary neurons.
来自啮齿动物的原代神经元培养是神经生物学中的关键工具。然而，原代培养物的制备需要精确规划，从动物交配开始。此外，每次制备都会产生大量最终被浪费的细胞。冷冻保存原代神经细胞的可能性为研究提供了一种资源，并显著减少了动物的牺牲。在这里，我们描述了Neurostore缓冲液支持原代神经元的冷冻保存。
