Ichikawa Junya, Yamada Ryuji X, Muramatsu Rieko, Ikegaya Yuji, Matsuki Norio, Koyama Ryuta
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
J Pharmacol Sci. 2007 Aug;104(4):387-91. doi: 10.1254/jphs.sc0070162. Epub 2007 Aug 4.
Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals.
尽管神经元原代培养是细胞生物学和药理学研究的重要方法，但每次实验都必须牺牲许多动物。在此，我们引入一种新型系统来冷冻保存从新生大鼠制备的海马颗粒细胞（GCs）。在冷冻保存长达60天后解冻，GCs表达成熟神经元标志物MAP-2，并伸出单一的tau-1阳性轴突和多个tau-1阴性树突。这些特性与原代培养中的完整GCs非常相似，具有单次牺牲动物就能反复制备稳定培养物的优势。
