大鼠切除伤口愈合模型中伤口愈合事件的形态学评估
Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats.
作者信息
de Moura Estevão Lígia Reis, Cassini-Vieira Puebla, Leite Ana Greice Borba, de Carvalho Bulhões Apolônia Agnes Vilar, da Silva Barcelos Lucíola, Evêncio-Neto Joaquim
机构信息
Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil.
Department of Medicine, Division of Hematology/Oncology Department of Immunology and Microbiology, Cancer Center Rush University Medical Center, Chicago, USA.
出版信息
Bio Protoc. 2019 Jul 5;9(13):e3285. doi: 10.21769/BioProtoc.3285.
Abstract
Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we describe a protocol to perform macroscopic and microscopic analyses of the events that occur during skin wound healing using the experimental model of excisional wounds in rats.
摘要
皮肤伤口愈合是一个复杂的过程，涉及不同的事件，如血液凝固、炎症、新血管形成和细胞外基质沉积。这些事件可以通过组织学技术进行观察。然而，这些参数缺乏标准化会影响结果的可重复性。在此，我们描述了一种使用大鼠切除伤口实验模型对皮肤伤口愈合过程中发生的事件进行宏观和微观分析的方案。
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