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壳聚糖基生物材料联合间充质干细胞条件培养液在创面愈合和皮肤再生中的应用。

A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration.

机构信息

Department of Morphological Sciences, University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia.

Educational, Scientific and Research Institute AGEL, 811 06 Bratislava, Slovakia.

出版信息

Int J Mol Sci. 2023 Nov 8;24(22):16080. doi: 10.3390/ijms242216080.

DOI:10.3390/ijms242216080
PMID:38003269
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10671656/
Abstract

The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting.

摘要

本研究的目的是提供一种新型壳聚糖生物聚合物材料的有益治疗效果，该材料富含源自犬脂肪组织（AT-MSC）的间充质干细胞产物，用于兔模型的人工皮肤缺损。为了评估再生的客观性，我们使用了组织学分析和我们创建的评分系统，考虑到所有再生属性，如炎症反应、坏死、肉芽形成、单个皮肤层和毛囊的形成。与阴性对照（无生物材料的自发愈合）相比，我们观察到在八周和十周后，人工创建的皮肤缺损的愈合得到了加速和改善。此外，我们能够在移植后第八周用壳聚糖基生物材料处理的组织学皮肤样本中描述毛囊和表皮层。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf0/10671656/bec4556381ce/ijms-24-16080-g008.jpg
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