High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain.

Activation of type 1 cannabinoid (CB) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (, CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB receptors in the brain. hybridization and immunohistochemical techniques have been crucial for defining the CB receptor expression and localization at the cellular level. Nevertheless, more advanced methods are needed to reveal the precise topography of CB receptors in the brain, especially in unsuspected sites such as other cell types and organelles with low receptor expression (, glutamatergic neurons, astrocytes, mitochondria). High-resolution immunoelectron microscopy provides a more precise detection method for the subcellular localization of CB receptors in the brain. Herein, we describe a single pre-embedding immunogold method for electron microscopy based on the use of specific CB receptor antibodies and silver-intensified 1.4 nm gold-labeled Fab' fragments, and a combined pre-embedding immunogold and immunoperoxidase method that employs biotinylated secondary antibodies and avidin-biotin-peroxidase complex for the simultaneous localization of CB receptors and protein markers of specific brain cells or synapses (, GFAP, GLAST, IBA-1, PSD-95, gephyrin). In addition, a post-embedding immunogold method is also described and compared to the pre-embedding labeling procedure. These methods provide a relatively easy and useful approach for revealing the subcellular localization of low amounts of CB receptors in glutamatergic synapses, astrocytes, neuronal and astrocytic mitochondria in the brain.