髓鞘少突胶质细胞糖蛋白35-55（MOG 35-55）诱导的实验性自身免疫性脑脊髓炎：一种慢性多发性硬化症模型
Myelin Oligodendrocyte Glycoprotein 35-55 (MOG 35-55)-induced Experimental Autoimmune Encephalomyelitis: A Model of Chronic Multiple Sclerosis.
作者信息
Miyamura Sakie, Matsuo Nagisa, Nagayasu Kazuki, Shirakawa Hisashi, Kaneko Shuji
机构信息
Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
出版信息
Bio Protoc. 2019 Dec 20;9(24):e3453. doi: 10.21769/BioProtoc.3453.
Abstract
Multiple sclerosis (MS) is the common demyelinating disease of human central nervous system. Among mouse models available to study MS, including the cuprizone application and lysolecithin-injection models, experimental autoimmune encephalomyelitis (EAE) model is widely used so that chronic EAE model of C57BL/6J can reflect the autoimmune pathogenesis of MS well. Here we introduce the EAE model based on C57BL/6J mice, which is generated by injection of myelin oligodendrocyte glycoprotein 35-55 (MOG 35-55) as an antigen. After immunization with complete Freund's adjuvant, clinical signs and changes in body weight are observed one or two weeks later. The EAE model will continue to be useful for development of therapeutics for MS.
摘要
多发性硬化症（MS）是人类中枢神经系统常见的脱髓鞘疾病。在可用于研究MS的小鼠模型中，包括铜螯合剂应用模型和溶血卵磷脂注射模型，实验性自身免疫性脑脊髓炎（EAE）模型被广泛使用，因此C57BL/6J慢性EAE模型能够很好地反映MS的自身免疫发病机制。在此我们介绍基于C57BL/6J小鼠的EAE模型，该模型通过注射髓鞘少突胶质细胞糖蛋白35-55（MOG 35-55）作为抗原来构建。用完全弗氏佐剂免疫后，在一到两周后观察临床症状和体重变化。EAE模型将继续有助于MS治疗方法的开发。
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