Tezuka Takeaki, Nakane Daisuke, Kimura Tomohiro, Ohnishi Yasuo
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
Bio Protoc. 2019 Dec 20;9(24):e3458. doi: 10.21769/BioProtoc.3458.
Spherical zoospores of a rare actinomycete, , adhere to various hydrophobic solid surfaces by means of type IV pili. The purpose of this protocol is to provide detailed descriptions of the preparation of zoospores and an assay for the adhesion of the zoospores to solid surfaces. This adhesion assay, which measures numbers of zoospores that adhered to the dish surface and swimming zoospores in a tunnel chamber by using a phase-contrast microscope, can also be used for swimming cells of other microorganisms.
一种罕见放线菌的球形游动孢子通过IV型菌毛附着在各种疏水固体表面。本实验方案旨在详细描述游动孢子的制备方法以及游动孢子与固体表面黏附的检测方法。这种黏附检测方法通过相差显微镜测量附着在培养皿表面的游动孢子数量以及在隧道室中游动的游动孢子数量，也可用于其他微生物的游动细胞。
