Hashimoto Kyoko, Sato Shingo, Ochi Hiroki, Takeda Shu, Futakuchi Mitsuru
Department of Physiology and Cell Biology, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.
Department of Orthopaedic Surgery, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.
Bio Protoc. 2019 Feb 5;9(3):e3151. doi: 10.21769/BioProtoc.3151.
Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal models that mimic the behavior of tumor cells in bone metastatic lesions. Calvarial implantation model (bone invasion model) is a newly-established animal model that accurately recapitulates the behavior of tumor cells in the tumor-bone microenvironment. The surgical technique for tumor cell implantation is simpler than intracardiac, intra-arterial, or intraosseous injection techniques. This model can be useful for the identification of key factors driving tumor-induced osteolytic or osteoblastic changes.
骨是多种癌症常见的转移部位之一。在骨转移微环境中，肿瘤与骨的相互作用在溶骨性或成骨性骨转移的调控中起着重要作用。为了研究肿瘤细胞与骨组织之间的直接相互作用，建立能够模拟肿瘤细胞在骨转移病灶中行为的合适动物模型至关重要。颅骨植入模型（骨侵袭模型）是一种新建立的动物模型，能准确再现肿瘤细胞在肿瘤 - 骨微环境中的行为。肿瘤细胞植入的手术技术比心内、动脉内或骨内注射技术更简单。该模型有助于识别驱动肿瘤诱导的溶骨性或成骨性变化的关键因素。
