Joyce-Shaikh Barbara, Cua Daniel J, Bauché David
Merck & Co., Inc., MRL, Palo Alto, CA 94304-1104, USA.
Bio Protoc. 2019 Feb 5;9(3):e3153. doi: 10.21769/BioProtoc.3153.
Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (, DSS, TNBS) or pathogen-induced () have been used to study mechanisms involved in the development and regulation of colitis. Anti-CD40 induced colitis model has gained acceptance to study the roles of innate immune cells during acute intestinal inflammation. Here we describe a rapid, robust and reproducible protocol to induce and analyze anti-CD40 mediated colitis in mice.
结肠炎症或结肠炎在全球影响着超过100万人。包括化学诱导（如葡聚糖硫酸钠、三硝基苯磺酸）或病原体诱导（具体病原体未提及）在内的多种临床前模型已被用于研究结肠炎发生和调控所涉及的机制。抗CD40诱导的结肠炎模型已被认可用于研究急性肠道炎症期间固有免疫细胞的作用。在此，我们描述一种快速、可靠且可重复的方案，用于在小鼠中诱导和分析抗CD40介导的结肠炎。
