环状二鸟苷单磷酸（CDG）佐剂蛋白亚单位疫苗对黏膜和全身疫苗反应的评估
Evaluation of Mucosal and Systemic Vaccine Responses by Cyclic di-GMP (CDG)-adjuvanted Protein Subunit Vaccines.
作者信息
Mansouri Samira, Jin Lei
机构信息
Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Florida, Gainesville, FL, USA.
出版信息
Bio Protoc. 2019 Apr 20;9(8):e3217. doi: 10.21769/BioProtoc.3217.
Abstract
Intranasal administration of vaccine adjuvants directly deliver therapeutic agents to the lungs to induce potent lung mucosal immune responses. Cyclic di-GMP (CDG) is a promising mucosal vaccine adjuvant candidate capable of inducing protective immunity. This protocol describes an approach to induce and detect mucosal (lung) and systemic (blood and spleen) vaccine adjuvant responses of CDG. This protocol also includes the methods to detect both humoral and cellular immune responses of CDG adjuvant. Last, this protocol can be used to study other cyclic dinucleotides as mucosal vaccine adjuvants.
摘要
经鼻内给药的疫苗佐剂可将治疗剂直接递送至肺部，以诱导强大的肺黏膜免疫反应。环二鸟苷酸（CDG）是一种有前景的黏膜疫苗佐剂候选物，能够诱导保护性免疫。本方案描述了一种诱导和检测CDG的黏膜（肺）和全身（血液和脾脏）疫苗佐剂反应的方法。本方案还包括检测CDG佐剂的体液免疫和细胞免疫反应的方法。最后，本方案可用于研究其他环二核苷酸作为黏膜疫苗佐剂。
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本文引用的文献
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J Immunol. 2014 Jan 1;192(1):492-502. doi: 10.4049/jimmunol.1301812. Epub 2013 Dec 4.
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