Yan Hongbin, KuoLee Rhonda, Tram Kha, Qiu Hongyu, Zhang Jianbing, Patel Girishchandra B, Chen Wangxue
Department of Chemistry, Brock University, St. Catharines, Ontario, Canada.
Biochem Biophys Res Commun. 2009 Sep 25;387(3):581-4. doi: 10.1016/j.bbrc.2009.07.061. Epub 2009 Jul 16.
3',5'-Cyclic diguanylic acid (cdiGMP) is emerging as a universal bacterial second messenger in regulating bacterial growth on surfaces. It has been recently shown that cdiGMP stimulates innate immunity and enhances antigen-specific humoral and cellular immune responses. We herein report that intranasal (i.n.) administration with cdiGMP induces an acute but transient inflammatory response and activation of dendritic cells in the lungs. Moreover, i.n. immunization of mice with pneumococcal surface adhesion A (PsaA) in conjunction with cdiGMP elicited strong antigen-specific serum immunoglobulin G (IgG) and secretory IgA antibody responses at multiple mucosal surfaces. More importantly, the immunized mice showed significantly reduced nasopharyngeal Streptococcus pneumoniae colonization. These results, for the first time, provide direct evidence for the induction of protection against mucosal bacterial infections by cdiGMP as an adjuvant.
3',5'-环二鸟苷酸（cdiGMP）作为一种通用的细菌第二信使，在调节细菌在表面的生长过程中逐渐崭露头角。最近的研究表明，cdiGMP可刺激先天免疫，并增强抗原特异性体液免疫和细胞免疫反应。我们在此报告，经鼻内（i.n.）给予cdiGMP会在肺部引发急性但短暂的炎症反应以及树突状细胞的激活。此外，将肺炎球菌表面黏附素A（PsaA）与cdiGMP联合经鼻内免疫小鼠，会在多个黏膜表面引发强烈的抗原特异性血清免疫球蛋白G（IgG）和分泌型IgA抗体反应。更重要的是，免疫后的小鼠鼻咽部肺炎链球菌定植显著减少。这些结果首次为cdiGMP作为佐剂诱导针对黏膜细菌感染的保护作用提供了直接证据。
