Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.
Braz J Med Biol Res. 2021 Feb 26;54(4):e9850. doi: 10.1590/1414-431X20209850. eCollection 2021.
Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection.
呼吸道合胞病毒（RSV）感染是儿童下呼吸道感染的主要原因。然而，目前尚无针对 RSV 感染的有效治疗方法。在这里，我们旨在确定潜在的生物标志物，以辅助 RSV 感染的治疗。招募 RSV 感染急性期和恢复期的儿童，并进行蛋白质组学分析以鉴定差异表达蛋白（DEPs）。随后，通过功能富集和蛋白质-蛋白质相互作用网络分析确定有前途的候选蛋白，并在临床和小鼠模型样本中通过 Western blot 进一步验证。在 RSV 患者样本中鉴定出的 79 个 DEPs 中，有 4 种蛋白（BPGM、TPI1、PRDX2 和 CFL1）在 RSV 感染期间被证实显著上调。功能分析表明，BPGM 和 TPI1 主要参与糖酵解，表明 RSV 感染与糖酵解代谢途径有关。我们的研究结果提供了 RSV 感染期间蛋白质组图谱的见解，并表明 BPGM、TPI1、PRDX2 和 CFL1 可能是 RSV 感染治疗的潜在治疗生物标志物或靶标。
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