Yu Xinfen, Kou Yu, Xia Daozong, Li Jun, Yang Xuhui, Zhou Yinyan, He Xiaoyan
Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, China.
Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, China.
J Clin Virol. 2015 Aug;69:1-6. doi: 10.1016/j.jcv.2015.05.015. Epub 2015 May 21.
Human respiratory syncytial virus (RSV) is the most important viral pathogen in children. However, its epidemic patterns and co-infection characteristics are not fully understood.
We attempted to determine the level of genetic variation of RSV, and describe the prevalence and co-infection characteristics of RSV in Hangzhou during two epidemic seasons.
Single respiratory samples from 1820 pediatric patients were screened for RSV and genotyped by RT-PCR and sequencing. In all RSV positive specimens, we screened for viruses and atypical bacteria. Demographic and clinical information was recorded and analyzed.
A total of 34.5% and 3.8% of samples from acute lower respiratory tract infections (ALRI) and influenza-like illness (ILI) were positive for RSV, respectively. Phylogenetic analysis revealed that 61.1% of the selected 167 RSV strains were NA1, 31.1% were BA, 3.6% were ON1, 2.4% were CB1, and 1.8% were NA3. A new genotype, BA11 was identified, which comprised 98.1% of BA strains in this study, while the rest were BA10. A total of 36.4% and 9.1% of RSV-positive children with ALRI and ILI respectively were found to be co-infected. Rhinovirus was the most common additional respiratory virus, followed by human metapneumovirus. Except for fever, no significant differences in other clinical presentation between the RSV mono-infection and co-infection groups were observed.
The circulating RSV strains had high genetic variability with RSV-B showing a more local pattern. In ALRI cases, co-infection of RSV with other viruses or atypical bacteria has no significant effect on the clinical presentation except fever.
人呼吸道合胞病毒（RSV）是儿童最重要的病毒病原体。然而，其流行模式和合并感染特征尚未完全明确。
我们试图确定RSV的基因变异水平，并描述杭州两个流行季节RSV的流行情况及合并感染特征。
对1820例儿科患者的单份呼吸道样本进行RSV筛查，并通过逆转录聚合酶链反应（RT-PCR）和测序进行基因分型。在所有RSV阳性标本中，我们筛查了病毒和非典型细菌。记录并分析人口统计学和临床信息。
急性下呼吸道感染（ALRI）和流感样疾病（ILI）样本中分别有34.5%和3.8%的RSV呈阳性。系统发育分析显示，在所选的167株RSV毒株中，61.1%为NA1型，31.1%为BA型，3.6%为ON1型，2.4%为CB1型，1.8%为NA3型。鉴定出一种新的基因型BA11，在本研究中占BA毒株的98.1%，其余为BA10型。ALRI和ILI中分别有36.4%和9.1%的RSV阳性儿童被发现合并感染。鼻病毒是最常见的其他呼吸道病毒，其次是人偏肺病毒。除发热外，RSV单一感染组和合并感染组在其他临床表现上未观察到显著差异。
循环中的RSV毒株具有高度的基因变异性，RSV-B呈现出更具局部性的模式。在ALRI病例中，RSV与其他病毒或非典型细菌的合并感染除发热外对临床表现无显著影响。
