Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG, Rio Grande, Brazil.
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas-UFPEL, Capão do Leão, Brazil.
Zebrafish. 2021 Apr;18(2):139-148. doi: 10.1089/zeb.2020.1950. Epub 2021 Mar 2.
Many scientific studies still use zebrafish from pet stores as animal models, even cutting-edge researches. However, these animals differ genotypically and phenotypically between them. The importance of the use of standardized models is widely recognized. Besides that, another consequence of using zebrafish from unknown origins is the acquisition of parasitized animals. This study aimed to relate the infection by sp. in zebrafish. Animals sold as "high standard" were acquired from a commercial company. Swimming alterations and superficial yellow dots were observed in five zebrafish with clinical signs, which were isolated, euthanized, and necropsied. Muscular yellow cysts with metacercaria associated with lesions were observed. The muscular cysts were responsible for the superficial yellow dots as well as the swimming alterations. The prevalence was 2.5%, and the mean infection intensity was 7 digeneans/host. The cysts measured a mean of 1251.43 μm long × 784.28 μm wide. Metacercariae measured a mean of 4847 μm long × 1353 μm wide. This first report about infection by sp. in zebrafish is globally relevant since the host and the parasite genus currently overlap worldwide. Furthermore, this study sheds light on the importance of the specific pathogen-free commercial creations or laboratory-reared zebrafish for research.
许多科学研究仍然使用宠物店的斑马鱼作为动物模型，甚至是前沿研究。然而，这些动物在基因型和表型上存在差异。使用标准化模型的重要性已得到广泛认可。此外，使用来源不明的斑马鱼的另一个后果是获得寄生虫感染的动物。本研究旨在探讨 sp. 感染斑马鱼的情况。从一家商业公司购买了被标记为“高标准”的动物。五只出现临床症状的斑马鱼出现游泳异常和体表黄色斑点，将其隔离、安乐死并进行剖检。观察到肌肉黄色囊肿伴有与病变相关的似囊尾蚴。肌肉囊肿是导致体表黄色斑点和游泳异常的原因。感染率为 2.5%，平均感染强度为 7 条双腔吸虫/宿主。囊肿的平均长度为 1251.43μm×784.28μm，宽 1251.43μm×784.28μm。似囊尾蚴的平均长度为 4847μm×1353μm。这是首次报道 sp. 感染斑马鱼，在全球范围内具有重要意义，因为宿主和寄生虫属目前在全球范围内重叠。此外，本研究强调了为研究目的使用无特定病原体的商业养殖或实验室养殖斑马鱼的重要性。
