利用杆状病毒表达系统在 Sf9 昆虫细胞中表达拟南芥隐花色素。
Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System.
机构信息
National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, P. R. China.
出版信息
Methods Mol Biol. 2021;2297:155-160. doi: 10.1007/978-1-0716-1370-2_16.
PMID:33656679
Abstract
The Bac-to-Bac Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.
摘要
杆状病毒-杆状病毒表达系统提供了一种快速有效的方法来产生具有辅因子黄素（FAD）的重组隐花色素（CRY）蛋白，该蛋白在体外表现出蓝光反应。
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