Department of Clinical Neurobiology at the Medical Faculty of Heidelberg University and German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Department of Clinical Neurobiology at the Medical Faculty of Heidelberg University and German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Cell Rep. 2021 Mar 2;34(9):108801. doi: 10.1016/j.celrep.2021.108801.
Septal parvalbumin-expressing (PV) and calbindin-expressing (CB) projections inhibit low-threshold and fast-spiking interneurons, respectively, in the medial entorhinal cortex (MEC). We investigate how the two inputs control neuronal activity in the MEC in freely moving mice. Stimulation of PV and CB terminals causes disinhibition of spatially tuned MEC neurons, but exerts differential effects on temporal coding and burst firing. Thus, recruitment of PV projections disrupts theta-rhythmic firing of MEC neurons, while stimulation of CB projections increases burst firing of grid cells and enhances phase precession in a cell-type-specific manner. Inactivation of septal PV or CB neurons differentially affects context, reference, and working memory. Together, our results reveal how specific connectivity of septal GABAergic projections with MEC interneurons translates into differential modulation of MEC neuronal coding.
隔区表达囊泡蛋白（PV）和钙结合蛋白（CB）的投射分别抑制内侧缰核皮质（MEC）中的低阈值和快速放电中间神经元。我们研究了这两种输入如何在自由活动的小鼠中控制 MEC 中的神经元活动。刺激 PV 和 CB 末梢会导致空间调谐的 MEC 神经元去抑制，但对时间编码和爆发放电有不同的影响。因此，PV 投射的募集会破坏 MEC 神经元的θ节律性放电，而 CB 投射的刺激则以细胞类型特异性的方式增加网格细胞的爆发放电并增强相位超前。隔区 PV 或 CB 神经元的失活会以不同的方式影响上下文、参照和工作记忆。总之，我们的结果揭示了隔区 GABA 能投射与 MEC 中间神经元的特定连接如何转化为 MEC 神经元编码的差异调节。
