Toth I K, Mulholland V, Cooper V, Bentley S, Shih Y-L, Perombelon M C M, Salmond G P C
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
Microbiology (Reading). 1997 Jul;143(7):2433-2438. doi: 10.1099/00221287-143-7-2433.
Using enrichment methods, a new bacteriophage (M1) was isolated, which is capable of generalized transduction in subsp. () strain SCRI1043. M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. M1 infected over 25% of strains tested and so may be useful both for the genetic analysis of a number of isolates and for the transductional transfer of selectable markers between strains.
通过富集方法，分离出一种新的噬菌体（M1），它能够在亚种（）菌株SCRI1043中进行广义转导。M1可能是一种烈性噬菌体，含有约43 kb的双链DNA。确定了多种染色体标记和质粒pHCP2的转导频率，并优化了转导条件。转导前对裂解物进行紫外线照射可提高转导频率。M1感染了超过25%的测试菌株，因此可能对多种分离株的遗传分析以及菌株间选择标记的转导转移都有用。
