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rsmS(ybaM)突变导致肠细菌植物病原菌中 RsmAB 转录后毒力调控系统的旁路抑制。

The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens.

机构信息

Department of Biochemistry, Hopkins Building, Tennis Court Road, University of Cambridge, Cambridge, CB2 1QW, UK.

Faculty of Medicine, School of Public Health, Imperial College, London, St Mary's Campus, Norfolk Place, W2 1PG, UK.

出版信息

Sci Rep. 2019 Mar 14;9(1):4525. doi: 10.1038/s41598-019-40970-3.

DOI:10.1038/s41598-019-40970-3
PMID:30872786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6418279/
Abstract

Plant cell wall degrading enzymes (PCWDEs) are the primary virulence determinants of soft rotting bacteria such as the potato pathogen, Pectobacterium atrosepticum. The regulation of secondary metabolite (Rsm) system controls production of PCWDEs in response to changing nutrient conditions. This work identified a new suppressor of an rsmB mutation - ECA1172 or rsmS (rsmB suppressor). Mutants defective in rsmB (encoding a small regulatory RNA), show reduced elaboration of the quorum sensing molecule (N-3-oxohexanoyl-homoserine lactone; OHHL) and PCWDEs. However, OHHL and PCWDE production were partially restored in an rsmB, rsmS double mutant. Single rsmS mutants, overproduced PCWDEs and OHHL relative to wild type P. atrosepticum and exhibited hypervirulence in potato. RsmS overproduction also resulted in increased PCWDEs and OHHL. Homology searches revealed rsmS conservation across pathogens such as Escherichia coli (ybaM), Dickeya solani, Klebsiella pneumoniae and Shigella flexneri. An rsmS mutant of Pectobacterium carotovorum ATCC39048 showed bypass of rsmB-dependent repression of PCWDEs and OHHL production. P. carotovorum ATCC39048 produces the β-lactam antibiotic, 1-carbapen-2-em-3-carboxylic acid (a carbapenem). Production of the antibiotic was repressed in an rsmB mutant but partially restored in an rsmB, rsmS double mutant. This work highlights the importance of RsmS, as a conserved pleiotropic regulator of virulence and antibiotic biosynthesis.

摘要

植物细胞壁降解酶（PCWDEs）是软腐菌主要的毒力决定因子，如马铃薯病原体果胶杆菌属（Pectobacterium）。次级代谢物（Rsm）系统调节 PCWDEs 的产生以响应不断变化的营养条件。本研究鉴定了一个新的 rsmB 突变抑制因子——ECA1172 或 rsmS（rsmB 抑制因子）。rsmB（编码一个小调控 RNA）缺陷突变体表现出群体感应分子（N-3-氧代己酰高丝氨酸内酯；OHHL）和 PCWDEs 产生减少。然而，在 rsmB、rsmS 双突变体中，OHHL 和 PCWDEs 的产生部分恢复。与野生型果胶杆菌属相比，rsmS 单突变体过度产生 PCWDEs 和 OHHL，并在马铃薯中表现出超毒性。rsmS 过表达也导致 PCWDEs 和 OHHL 的增加。同源搜索显示 rsmS 在病原体中具有保守性，如大肠杆菌（ybaM）、地衣芽孢杆菌、肺炎克雷伯菌和福氏志贺菌。果胶杆菌属（Pectobacterium）的 rsmS 突变体 ATCC39048 显示出绕过 rsmB 依赖性对 PCWDEs 和 OHHL 产生的抑制。果胶杆菌属 ATCC39048 产生β-内酰胺抗生素 1-碳青霉烯-2-Em-3-羧酸（一种碳青霉烯）。抗生素的产生在 rsmB 突变体中受到抑制，但在 rsmB、rsmS 双突变体中部分恢复。本研究强调了 RsmS 作为一个保守的多效性毒力和抗生素生物合成调节因子的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/4daa94742c06/41598_2019_40970_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/74ef0aeb129b/41598_2019_40970_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/8284e89d5755/41598_2019_40970_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/894319f50897/41598_2019_40970_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/fe11cac23f25/41598_2019_40970_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/4daa94742c06/41598_2019_40970_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/74ef0aeb129b/41598_2019_40970_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/ef6193256d31/41598_2019_40970_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/d318f50c01c5/41598_2019_40970_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/ffaa17400a57/41598_2019_40970_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/8284e89d5755/41598_2019_40970_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/894319f50897/41598_2019_40970_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/fe11cac23f25/41598_2019_40970_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d0/6418279/4daa94742c06/41598_2019_40970_Fig8_HTML.jpg

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