Basbas Carl, Byrne Barbara A, Chigerwe Munashe, Escobar Edlin D, Hodzic Emir, Pires Alda F A, Pereira Richard V
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Front Microbiol. 2021 Feb 15;12:601924. doi: 10.3389/fmicb.2021.601924. eCollection 2021.
The objectives of this study were to evaluate the prevalence of extended spectrum β-lactamase (ESBL) genes, AmpC-type β-lactamase (ACBL) genes, and plasmid mediated quinolone resistance (PMQR) genes in isolated at a Veterinary Medical Teaching Hospital microbiology laboratory, examine trends in presence of these resistance genes, and to explore the correlation between phenotypic resistance and presence of specific genes. The presence of ESBL, ACBL, and PMQR genes were detected using a single, novel multiplex qPCR. Only the genes and were detected in the 110 isolates tested. PMQR genes were not detected in isolates screened. Of 94 third-generation cephalosporin resistant isolates, representing eight serotypes, 48% ( = 45) were positive for only and 50% ( = 47) were simultaneously positive for and . Two third-generation cephalosporin resistant isolates were tested negative for all β-lactamase genes in our qPCR assay and likely house ESBL genes not screened for by our qPCR assay. A logistic regression model revealed that for serotype Dublin isolates ( = 38) the odds ratio for testing positive for when compared to all other serotypes was 51.6 (95% CI: 4.01-664.03, = 0.0029). For serotype Typhimurium ( = 9) the odds ratio for testing positive for when compared to all other serotypes was 43.3 (95% CI: 1.76-1000, = 0.0216). Overall, our results suggest that the prevalence of resistance to cephalosporins and fluoroquinolones due to ESBLs, ACBLs, and PMQR genes present in bovine nontyphoidal isolates has remained relatively constant in the isolates screened over a 14-year period.
本研究的目的是评估某兽医教学医院微生物实验室分离出的菌株中广谱β-内酰胺酶（ESBL）基因、AmpC型β-内酰胺酶（ACBL）基因和质粒介导喹诺酮耐药性（PMQR）基因的流行情况，研究这些耐药基因存在情况的变化趋势，并探讨表型耐药性与特定基因存在之间的相关性。使用一种新型的单一多重定量聚合酶链反应（qPCR）检测ESBL、ACBL和PMQR基因的存在情况。在所检测的110株菌株中，仅检测到了 基因和 基因。在所筛选的菌株中未检测到PMQR基因。在代表8种血清型的94株对第三代头孢菌素耐药的菌株中，48%（n = 45）仅 呈阳性，50%（n = 47） 和 同时呈阳性。在我们的qPCR检测中，有两株对第三代头孢菌素耐药的菌株所有β-内酰胺酶基因检测均为阴性，可能携带我们的qPCR检测未筛查的ESBL基因。逻辑回归模型显示，对于都柏林血清型菌株（n = 38），与所有其他血清型相比， 检测呈阳性的优势比为51.6（95%置信区间：4.01 - 664.03，P = 0.0029）。对于鼠伤寒血清型菌株（n = 9），与所有其他血清型相比， 检测呈阳性的优势比为43.3（95%置信区间：1.76 - 1000，P = 0.0216）。总体而言，我们的结果表明，在14年期间所筛选的牛非伤寒沙门氏菌菌株中，由ESBL、ACBL和PMQR基因导致的对头孢菌素和氟喹诺酮的耐药率相对保持稳定。
