γ-谷氨酰转移酶在小牛胸腺细胞中的亚细胞定位

Subcellular localization of gamma-glutamyltransferase in calf thymocytes.

作者信息

Suter D, Brunner G, Ferber E

出版信息

Biochem J. 1978 Nov 1;175(2):643-7. doi: 10.1042/bj1750643.

DOI:10.1042/bj1750643

PMID:33659

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186114/

Abstract

The subcellular localization of gamma-glutamyltransferase in calf thymocytes was investigated and compared with that of alkaline phosphodiesterase I, alkaline nitrophenyl phosphatase, succinate-tetrazolium oxidoreductase (succinate-INT reductase) and lactate dehydrogenase after two different methods of cell disruption and differential centrifugation. Most of the activity was recovered in the crude membrane fractions (43.0%), but significant amounts co-pelleted with the large-granule (mitochondria) fractions (31%). The specific activity of the gamma-glutamyltransferase in the purified plasma membrane was 30-50 times that of the enzyme in the cell homogenate and had a similar subcellular distribution to the plasma-membrane markers, alkaline phosphodiesterase I and alkaline nitrophenyl phosphatase. It was concluded that gamma-glutamyltransferase was primary a plasma-membrane-bound enzyme, and that its location in other subcellular fractions was probably due to their contamination with plasma-membrane vesicles.

摘要

研究了小牛胸腺细胞中γ-谷氨酰转移酶的亚细胞定位，并在两种不同的细胞破碎和差速离心方法后，将其与碱性磷酸二酯酶I、碱性硝基苯磷酸酶、琥珀酸-四氮唑氧化还原酶（琥珀酸-INT还原酶）和乳酸脱氢酶的亚细胞定位进行了比较。大部分活性在粗膜级分中回收（43.0%），但有相当数量的活性与大颗粒（线粒体）级分共沉淀（31%）。纯化质膜中γ-谷氨酰转移酶的比活性是细胞匀浆中该酶的30至50倍，并且其亚细胞分布与质膜标记物碱性磷酸二酯酶I和碱性硝基苯磷酸酶相似。得出的结论是，γ-谷氨酰转移酶主要是一种与质膜结合的酶，其在其他亚细胞级分中的定位可能是由于它们被质膜囊泡污染所致。

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本文引用的文献

1

Normal amino acid uptake by cultured human fibroblasts does not require gamma-glutamyl transpeptidase.培养的人成纤维细胞对正常氨基酸的摄取不需要γ-谷氨酰转肽酶。

Biochem Biophys Res Commun. 1976 Dec 20;73(4):997-1002. doi: 10.1016/0006-291x(76)90221-7.

2

Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.组织分级分离研究。5. 大鼠肝脏中酸性磷酸酶与一类特殊细胞质颗粒的关联。

Biochem J. 1955 Mar;59(3):438-45. doi: 10.1042/bj0590438.

3

Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.营养不良性肌肉的生物化学。线粒体琥珀酸 - 四氮唑还原酶和三磷酸腺苷酶。

Biochem J. 1961 Sep;80(3):649-54. doi: 10.1042/bj0800649.

4

Isolation of rat liver plasma membranes. Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes.大鼠肝细胞膜的分离。使用焦磷酸酶和磷酸二酯酶I作为标记酶。

J Cell Biol. 1970 Dec;47(3):604-18. doi: 10.1083/jcb.47.3.604.

5

Isolation and characterization of lymphocyte plasma membranes.淋巴细胞质膜的分离与鉴定

Biochim Biophys Acta. 1972 May 9;266(2):494-504. doi: 10.1016/0005-2736(72)90105-8.

6

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Contemp Top Mol Immunol. 1974;3:27-56. doi: 10.1007/978-1-4684-2838-4_2.

7

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Biochem J. 1973 Dec;135(4):819-26. doi: 10.1042/bj1350819.

8

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Biochem J. 1970 Nov;120(1):133-43. doi: 10.1042/bj1200133.

9

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Arch Biochem Biophys. 1968 Oct;128(1):51-69. doi: 10.1016/0003-9861(68)90008-8.

10

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