Cara Nicolás, Marfil Carlos F, Bertoldi María V, Masuelli Ricardo W
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza (IBAM). Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Almirante Brown 500. Chacras de Coria. Mendoza. M5528AHB. Argentina.
Bio Protoc. 2020 Jul 5;10(13):e3671. doi: 10.21769/BioProtoc.3671.
Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, the inheritance of specific methylation patterns can be studied. Here, we present a protocol for analyzing DNA methylation patterns through MSAP markers in potato interspecific hybrids and their parental genotypes.
甲基化敏感扩增多态性（MSAP）是一种用于分析非模式物种DNA甲基化模式的通用标记。该技术的实施不需要参考基因组，并且能够确定分布在整个基因组中的数百个匿名位点的甲基化状态。此外，还可以研究特定甲基化模式的遗传情况。在此，我们展示了一种通过MSAP标记分析马铃薯种间杂种及其亲本基因型DNA甲基化模式的方案。
