Arbués Ainhoa, Kammüller Michael, Portevin Damien
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.
Bio Protoc. 2020 Nov 20;10(22):e3820. doi: 10.21769/BioProtoc.3820.
Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen A better understanding of the complex host- interactions within the granuloma's environment may lead to new therapeutic or preventive tools to improve the control of the tuberculosis pandemic. To date, several models that are able to mimic human nascent granulomas have been reported. Here we describe a protocol in which -infected human peripheral blood mononuclear cells (PBMCs) are embedded within a collagen matrix leading to the formation of three-dimensional micro-granulomas. Subsequently, PBMCs and can be retrieved allowing multiparametric readouts from both the host and the pathogen. In addition to the incorporation of a physiological extracellular matrix, this model has the singular advantage of recapitulating dormant-like features, as well as reproducing resuscitation observed under immunomodulatory treatments, which have not been reported in other published protocols to generate granulomas.
肉芽肿是有组织的多细胞结构，是人类病原体感染的标志。更好地理解肉芽肿环境中复杂的宿主相互作用，可能会带来新的治疗或预防手段，以改善对结核病大流行的控制。迄今为止，已经报道了几种能够模拟人类新生肉芽肿的模型。在此，我们描述一种方案，即将感染的人类外周血单核细胞（PBMC）嵌入胶原蛋白基质中，从而形成三维微肉芽肿。随后，可以获取PBMC和病原体，以便对宿主和病原体进行多参数读数。除了包含生理细胞外基质外，该模型具有独特的优势，即能够重现类似休眠的特征，以及再现免疫调节治疗下观察到的复苏情况，而在其他已发表的生成肉芽肿的方案中尚未报道过这些情况。
