Chae Song Ah, Son Jun Seok, Zhu Mei-Jun, De Avila Jeanene M, Du And Min
Nutrigenomics and Growth Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
School of Food Science, Washington State University, Pullman, WA 99164, USA.
Bio Protoc. 2020 Dec 5;10(23):e3838. doi: 10.21769/BioProtoc.3838.
Epidemiological studies robustly show the beneficial effects of maternal exercise in reducing maternal birth complications and improving neonatal outcomes, though underlying mechanisms remain poorly understood. To facilitate mechanistic exploration, a protocol for maternal exercise of mice is established, with the regimen following the exercise guidelines for pregnant women. Compared to volunteer wheel running, treadmill running allows precise control of exercise intensity and duration, dramatically reducing variations among individual mouse within treatments and facilitating translation into maternal exercise in humans. Based on the maximal oxygen consumption rate (VOmax) before pregnancy, the treadmill exercise protocol is separated into three stages: early stage (E1.5 to E7.5 at 40% VOmax), mid stage (E8.5 to E14.5 at 65% VOmax), and late stage of pregnancy (E15.5 to birth at 50% VOmax), which demonstrated persistent beneficial effects on maternal health and fetal development. This protocol can be useful for standardizing maternal treadmill exercise using mice as an experimental model.
流行病学研究有力地表明，孕期运动对减少产妇分娩并发症和改善新生儿结局具有有益作用，但其潜在机制仍知之甚少。为便于进行机制探索，我们建立了一套小鼠孕期运动方案，该方案遵循孕妇运动指南。与自愿转轮运动相比，跑步机跑步能精确控制运动强度和持续时间，极大地减少了各治疗组内个体小鼠之间的差异，并有助于转化为人类的孕期运动。根据怀孕前的最大耗氧率（VOmax），跑步机运动方案分为三个阶段：早期（妊娠第1.5天至第7.5天，运动强度为VOmax的40%）、中期（妊娠第8.5天至第14.5天，运动强度为VOmax的65%）和妊娠后期（妊娠第15.5天至分娩，运动强度为VOmax的50%），该方案对母体健康和胎儿发育具有持续的有益影响。该方案有助于将小鼠作为实验模型，规范孕期跑步机运动。
