HIV-CRISPR：一种用于鉴定影响HIV复制基因的CRISPR/Cas9筛选方法。
HIV-CRISPR: A CRISPR/Cas9 Screening Method to Identify Genes Affecting HIV Replication.
作者信息
Roesch Ferdinand, OhAinle Molly
机构信息
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, USA.
Department of Virology, Institut Pasteur, Paris, France.
出版信息
Bio Protoc. 2020 May 5;10(9):e3614. doi: 10.21769/BioProtoc.3614.
Abstract
Screening with CRISPR/Cas9 technology has already led to significant discoveries in the fields of cancer biology, cell biology and virology. Because of the relatively low false discovery rates and the ability to perform high-throughput, pooled approaches, it has rapidly become the assay of choice for screening studies, including whole-genome screens. Here, we describe a CRISPR screening protocol that allows for efficient screening of the entire life cycle of HIV-1 through packaging of the HIV-CRISPR lentiviral genomes by infecting HIV-1 virus .
摘要
利用CRISPR/Cas9技术进行筛选已在癌症生物学、细胞生物学和病毒学领域带来了重大发现。由于相对较低的假阳性率以及能够进行高通量的混合方法，它迅速成为包括全基因组筛选在内的筛选研究的首选检测方法。在此，我们描述了一种CRISPR筛选方案，该方案通过感染HIV-1病毒包装HIV-CRISPR慢病毒基因组，从而能够对HIV-1的整个生命周期进行高效筛选。
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