Kundu Shantanu, Tyagi Kaomud, Alam Imran, Maheswaran Gopinathan, Kumar Vikas, Chandra Kailash
Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata, India.
Bird Section, Zoological Survey of India, Kolkata, India.
Mitochondrial DNA B Resour. 2021 Feb 8;6(2):339-343. doi: 10.1080/23802359.2020.1866448.
The complete mitogenome sequence of the brown-headed gull, was determined in this study. The 16,771 bp genome consists of 13 protein-coding genes (PCGs), two ribosomal RNA () genes, and 22 transfer RNA () genes, and a control region (CR). The decoded mitogenome was AT-rich (54.77%) with nine overlapping and 17 intergenic spacer regions. Most of the PCGs were started by a typical ATG initiation codon except for and . Further, the usual termination codons (AGG, TAG, TAA, and AGA) were used by 11 PCGs except for and . The concatenated PCGs based Bayesian phylogeny clearly discriminates all the Laridae species and reflects the sister relationship of with . The present mitogenome-based phylogeny was congruent with the earlier hypothesis and confirmed the evolutionary position of the brown-headed gull as masked species. The generated mitogenome of is almost identical to the previously generated mitogenome from China except for two base pairs in CR. To visualize the population structure of this migratory species, we propose more sampling from different geographical locations and the generation of additional molecular data to clarify the reality.
本研究测定了棕头鸥的完整线粒体基因组序列。该16771bp的基因组由13个蛋白质编码基因（PCGs）、两个核糖体RNA（rRNA）基因、22个转运RNA（tRNA）基因和一个控制区（CR）组成。解码后的线粒体基因组富含AT（54.77%），有9个重叠区和17个基因间隔区。除了COX1和ND2外，大多数PCGs由典型的ATG起始密码子起始。此外，除了COX1和ND6外，11个PCGs使用常见的终止密码子（AGG、TAG、TAA和AGA）。基于串联PCGs的贝叶斯系统发育清楚地区分了所有鸥科物种，并反映了棕头鸥与渔鸥的姐妹关系。目前基于线粒体基因组的系统发育与早期假设一致，并确认了棕头鸥作为隐存物种的进化地位。除了控制区的两个碱基对外，本研究生成的棕头鸥线粒体基因组与之前来自中国的线粒体基因组几乎相同。为了可视化这种迁徙物种的种群结构，我们建议从不同地理位置进行更多采样，并生成更多分子数据以阐明实际情况。
