Terasaki Momoka, Kimura Yukiko, Yamada Masato, Nishida Hiromi
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
Narimasa Sake Brewery, 418 Tachi, Nanto, Toyama 939-1676, Japan.
AIMS Microbiol. 2021 Feb 26;7(1):114-123. doi: 10.3934/microbiol.2021008. eCollection 2021.
Bacteria belonging to the genus were identified as bacteria peculiar to a sake brewery in Toyama, Japan. Comparison of the 16S rRNA gene sequences revealed two groups of isolates. Among known species, one group was similar to ( type), and the other, ( type). We determined complete genomic DNA sequences from two isolates, TGY1120_3 and TGY1127_2, which belong to types and , respectively. Comparison of these genomic information showed that these isolates differ at the species level with different genomic characters. Isolate TGY1120_3 comprised one chromosome and three plasmids, and the same transposon coding region was located on two loci on the chromosome and one locus on one plasmid, suggesting that the genetic element may be transferred between the chromosome and plasmid. Isolate TGY1127_2 comprised one chromosome and one plasmid. This plasmid encoded an identical transposase coding region, strongly suggesting that the genetic element may be transferred between these different isolates through plasmids. These four plasmids carried a highly similar region, indicating that they share a common ancestor. Thus, these two isolates may form a community and exchange their genetic information during sake brewing.
属于该属的细菌被鉴定为日本富山一家清酒酿造厂特有的细菌。16S rRNA基因序列比较揭示了两组分离株。在已知物种中，一组与（型）相似，另一组与（型）相似。我们测定了分别属于型和型的两个分离株TGY1120_3和TGY1127_2的完整基因组DNA序列。这些基因组信息的比较表明，这些分离株在物种水平上存在差异，具有不同的基因组特征。分离株TGY1120_3由一条染色体和三个质粒组成，相同的转座子编码区位于染色体上的两个位点和一个质粒上的一个位点，这表明该遗传元件可能在染色体和质粒之间转移。分离株TGY1127_2由一条染色体和一个质粒组成。该质粒编码一个相同的转座酶编码区，强烈表明该遗传元件可能通过质粒在这些不同的分离株之间转移。这四个质粒携带一个高度相似的区域，表明它们有共同的祖先。因此，这两个分离株可能形成一个群落，并在清酒酿造过程中交换它们的遗传信息。
