在塔吉克斯坦采集的安纳托利亚璃眼蜱中检测埃立克体属和泰勒虫属。

Detection of Ehrlichia spp. and Theileria spp. in Hyalomma anatolicum ticks collected in Tajikistan.

作者信息

Kartashov M Yu, Kononova Yu V, Petrova I D, Tupota N L, Mikryukova T P, Ternovoi V A, Tishkova F H, Loktev V B

机构信息

State Research Center for Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, Russia Novosibirsk State University, Novosibirsk, Russia.

State Research Center for Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, Russia Tomsk State University, Tomsk, Russia.

出版信息

Vavilovskii Zhurnal Genet Selektsii. 2020 Feb;24(1):55-59. doi: 10.18699/VJ20.595.

DOI:10.18699/VJ20.595

PMID:33659781

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7716523/

Abstract

The objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. and fragments of the 18S rRNA gene of Theileria spp. detected in H. anatolicum ticks. Hyalomma anatolicum ticks collected in the Tursunzade and Rudaki districts of Tajikistan were tested for DNA of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. by PCR with specific primers. The amplified fragments were sequenced and analyzed. DNA of Ehrlichia spp. (3.3 %) and Theileria spp. (3.3 %) was detected only in H. anatolicum ticks collected from the Rudaki district, and DNA of Ehrlichia spp. (0.7 %) was found in H. anatolicum ticks from the Tursunzade district. Sequence analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. revealed high similarity to Ehrlichia spp. The Tajik isolates of Theileria spp. were genotyped as Theileria annulata based on the analysis of 18S rRNA gene sequences. The phylogenetic analysis demonstrates that Ehrlichia spp. isolates are highly similar to Ehrlichia spp. circulating in China and Brazil. The isolate Tajikistan-5 is closely related to the putative novel species Ehrlichia mineirensis. The Tajik isolates of Theileria spp. were clustered with T. annulata isolates from Turkey, Iran, Pakistan, and China by phylogenetic analyses.

摘要

我们研究的目的是调查在塔吉克斯坦西南部采集的安纳托利亚璃眼蜱中，立克次体属、埃立克体属、无形体属、巴贝斯虫属和泰勒虫属的遗传标记的流行情况，并对在安纳托利亚璃眼蜱中检测到的埃立克体属的16S rRNA基因和groESL操纵子片段以及泰勒虫属的18S rRNA基因片段进行测序和系统发育分析。对在塔吉克斯坦图尔孙扎德和鲁达基地区采集的安纳托利亚璃眼蜱，使用特异性引物通过PCR检测立克次体属、埃立克体属、无形体属、巴贝斯虫属和泰勒虫属的DNA。对扩增片段进行测序和分析。仅在从鲁达基地区采集的安纳托利亚璃眼蜱中检测到埃立克体属（3.3%）和泰勒虫属（3.3%）的DNA，在图尔孙扎德地区的安纳托利亚璃眼蜱中发现了埃立克体属（0.7%）的DNA。对埃立克体属的16S rRNA基因和groESL操纵子片段的序列分析显示与埃立克体属高度相似。基于18S rRNA基因序列分析，塔吉克斯坦的泰勒虫属分离株被基因分型为环形泰勒虫。系统发育分析表明，埃立克体属分离株与在中国和巴西传播的埃立克体属高度相似。分离株塔吉克斯坦-5与假定的新物种米内罗埃立克体密切相关。通过系统发育分析，塔吉克斯坦的泰勒虫属分离株与来自土耳其、伊朗、巴基斯坦和中国的环形泰勒虫分离株聚类在一起。