Li Caishan, Wen Licui, Shi Wenyu, Deng Yuqian, Zhou Na, Zhao Xueqing, Guo Qingyong, Gailike Bayinchahan
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
Xinjiang Key Laboratory of New Drug Research and Development for Herbivores, Urumqi 830052, China.
Int J Mol Sci. 2025 Sep 1;26(17):8489. doi: 10.3390/ijms26178489.
The cytochrome p450 gene family is widely involved in various biological processes in arthropods. Tick p450s are often associated with chemical acaricides, but knowledge of their involvement in the metabolism of plant-derived essential oil components is limited. In this study, we identified the non-redundant number of p450 transcripts (NRNPTs) from and under the essential oil (CCEO) and terpinolene stress using de novo transcriptome data, respectively. In this study, we identified and characterized the NRNPTs of and . Their gene expression patterns and biological functions under CCEO and terpinolene stress were further analyzed. Finally, NRNPTs (87) were more numerous than (58). Phylogenetic analyses showed that NRNPTs of both and could be categorized in clan 2, clan 3, clan 4, and clan mito, this data comes from the NRNPTs. Phylogenetic analyses showed that NRNPTs of both and could be categorized in clan 2, clan 3, clan 4, and clan mito. p450 members of both were most distributed in clan 3. In addition, one NRNPT was identified as belonging to the new classification clan 20 (CYP20A1). The biological functions and pathways of p450 family members enriched in and under different exogenous substance stresses were different, and the expression patterns of these genes were inconsistent. Molecular docking results showed that p450 members (CYP3A4 and CYP4B1), which were significantly up-regulated under CCEO stress, as well as CYP24A1 and CYP4V2 (the CYP3A4 and CYP4B1 homologous genes), encode proteins that differ in their ability to metabolize CCEO components, but they all bind well to Germacrene D and naphthalene. Our study enriches the knowledge of the involvement of p450 family members of different tick species in the metabolism of essential oil components of plants, and provides a theoretical basis for further in-depth studies on the function of tick p450 enzymes.
细胞色素P450基因家族广泛参与节肢动物的各种生物过程。蜱类的P450通常与化学杀螨剂有关，但它们在植物源精油成分代谢中的作用了解有限。在本研究中，我们分别利用从头转录组数据，在香芹酚精油（CCEO）和萜品烯胁迫下，鉴定了[蜱种1]和[蜱种2]的非冗余P450转录本数量（NRNPTs）。在本研究中，我们鉴定并表征了[蜱种1]和[蜱种2]的NRNPTs。进一步分析了它们在CCEO和萜品烯胁迫下的基因表达模式和生物学功能。最后，[蜱种1]的NRNPTs（87个）比[蜱种2]（58个）更多。系统发育分析表明，[蜱种1]和[蜱种2]的NRNPTs均可归类于第2族、第3族、第4族和线粒体族，此数据来自NRNPTs。系统发育分析表明，[蜱种1]和[蜱种2]的NRNPTs均可归类于第2族、第3族、第4族和线粒体族。两者的P450成员大多分布在第3族。此外，一个[蜱种1]的NRNPT被鉴定属于新分类的第20族（CYP20A1）。在不同外源物质胁迫下，[蜱种1]和[蜱种2]中富集的P450家族成员的生物学功能和途径不同，这些基因的表达模式也不一致。分子对接结果表明，在CCEO胁迫下显著上调的[蜱种1]的P450成员（CYP3A4和CYP4B1），以及CYP24A1和CYP4V2（CYP3A4和CYP4B1的同源基因），编码的蛋白质在代谢CCEO成分的能力上有所不同，但它们都能很好地结合杜松烯D和萘。我们的研究丰富了不同蜱种P450家族成员参与植物精油成分代谢的知识，并为进一步深入研究蜱类P450酶的功能提供了理论依据。
