Department of Anesthesiology, Affiliated Hospital of Hebei University, Baoding, Hebei China.
Eur Rev Med Pharmacol Sci. 2021 Feb;25(4):2093-2098. doi: 10.26355/eurrev_202102_25114.
To investigate the effects of dexmedetomidine (Dex) treatment administered at various times on acute lung injury (ALI).
Thirty Wistar rats were randomly divided into five groups (n = 6/group). Lipopolysaccharide (LPS) was intraperitoneally injected into the rats in the LPS, Dex1, Dex2, and Dex3 groups to induce ALI, while the control group (C) was left untreated. Rats in the Dex1 group were intraperitoneally administered with 50 µg/kg Dex 30 minutes before modeling. Rats in the Dex2 group were injected with 25 µg/kg Dex 30 minutes before modeling and two hours after. Rats in the Dex3 group received 50 µg/kg Dex two hours after modeling. The animals in the C and LPS groups were given an equal volume of saline. The wet-to-dry (W/D) weight ratio of the rats' lungs was calculated, and pathological alterations in lung tissues were observed. The concentrations of inflammation-related factors and the expression of Janus kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), and matrix metallopeptidase 9 (MMP9) were measured.
The W/D ratio, expression of inflammatory factors, and expression of JAK1, STAT3, and MMP9 were significantly increased in the ALI rats (p < 0.05) compared with the C group. The level of anti-inflammatory factors in the Dex-treated groups was also significantly increased compared with the LPS group (p < 0.05). The concentration of anti-inflammatory factors in the Dex2 group was significantly higher than that recorded in the Dex1 and Dex3 groups (p < 0.05).
Dex treatment administered at different times protects rats against LPS-induced ALI to varying degrees. The protective effects of Dex were most robust when administered both before and after LPS stimulation.
研究不同时间给予右美托咪定（Dex）治疗对急性肺损伤（ALI）的影响。
30 只 Wistar 大鼠随机分为 5 组（每组 6 只）。LPS 组、Dex1 组、Dex2 组和 Dex3 组大鼠腹腔注射脂多糖（LPS）诱导 ALI，对照组（C 组）大鼠不做任何处理。Dex1 组大鼠在造模前 30 分钟腹腔注射 50μg/kg Dex，Dex2 组大鼠在造模前 30 分钟和 2 小时后分别注射 25μg/kg Dex，Dex3 组大鼠在造模后 2 小时给予 50μg/kg Dex。C 组和 LPS 组大鼠给予等体积生理盐水。计算大鼠肺组织的湿重/干重（W/D）比值，并观察肺组织的病理改变。检测炎症相关因子的浓度及 Janus 激酶 1（JAK1）、信号转导子和转录激活子 3（STAT3）和基质金属蛋白酶 9（MMP9）的表达。
与 C 组相比，ALI 大鼠的 W/D 比值、炎症因子表达及 JAK1、STAT3 和 MMP9 的表达显著增加（p<0.05）。与 LPS 组相比，Dex 治疗组抗炎因子水平也显著升高（p<0.05）。Dex2 组抗炎因子浓度显著高于 Dex1 组和 Dex3 组（p<0.05）。
不同时间给予 Dex 治疗可在不同程度上保护 LPS 诱导的 ALI 大鼠。在 LPS 刺激前后给予 Dex 的保护作用最强。
