Rontó G, Tóth K, Csik G, Feigin L A, Svergun D T, Dembo A T, Shtikova E V
Institute of Biophysics, Semmelweis Medical University, Budapest, Hungary.
Eur Biophys J. 1988;15(5):293-8. doi: 10.1007/BF00256480.
Structural parameters of phage T7 were compared in two frequently used Tris buffers of high and low ionic strength, in order to explain the different biological activity and drug-binding characteristics. Characteristics of the whole phage geometry were obtained by viscosimetry, static and quasi-elastic light-scattering and small-angle X-ray scattering. The latter method revealed dissimilarities in the intraphage DNA compactness, consistent with the findings of the optical absorption melting studies. Alterations in the particle dimensions determined in the same sample by different methods are discussed, and a model is constructed to explain the structural modifications that occur on lowering the ionic strength.
在两种常用的高离子强度和低离子强度的Tris缓冲液中比较了噬菌体T7的结构参数，以解释其不同的生物学活性和药物结合特性。通过粘度测定法、静态和准弹性光散射以及小角X射线散射获得了整个噬菌体几何结构的特征。后一种方法揭示了噬菌体内部DNA紧密程度的差异，这与光吸收熔解研究的结果一致。讨论了用不同方法在同一样品中测定的颗粒尺寸的变化，并构建了一个模型来解释降低离子强度时发生的结构修饰。
