Wang Hao, Yang Cheng, Tang Haoran, Li Yongxin
Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P. R. China.
Anal Chem. 2021 Mar 16;93(10):4593-4600. doi: 10.1021/acs.analchem.0c05055. Epub 2021 Mar 4.
Stochastic collision electrochemistry is a hot topic in single molecule/particle research, which provides an opportunity to investigate the details of the single molecule/particle reaction mechanism that is always masked in ensemble-averaged measurements. In this work, we develop an electrochemical amplification strategy to monitor the electrocatalytic behavior of single G-quadruplex/hemin (GQH) for the reaction between hydrogen peroxide and hydroquinone (HQ) through the collision upon a gold nanoelectrode. The intrinsic peroxidase activities of single GQH were investigated by stochastic collision electrochemical measurements, giving further insights into understanding biocatalytic processes. Based on the unique catalytic activity of GQH, we have also designed a hybridization chain reaction strategy to detect miRNA-15 with good selectivity and sensitivity. This work provided a meaningful strategy to investigate the electrochemical amplification and the broad application for nucleic acid sensing at the single molecule/particle level.
随机碰撞电化学是单分子/单颗粒研究中的一个热门话题,它为研究单分子/单颗粒反应机制的细节提供了契机,而这些细节在总体平均测量中往往被掩盖。在这项工作中,我们开发了一种电化学放大策略,通过在金纳米电极上的碰撞来监测单个G-四链体/血红素(GQH)对过氧化氢和对苯二酚(HQ)之间反应的电催化行为。通过随机碰撞电化学测量研究了单个GQH的固有过氧化物酶活性,为理解生物催化过程提供了进一步的见解。基于GQH独特的催化活性,我们还设计了一种杂交链式反应策略,以高选择性和高灵敏度检测miRNA-15。这项工作为在单分子/单颗粒水平上研究电化学放大及核酸传感的广泛应用提供了一种有意义的策略。