Dong Jingyi, Qiu Xia, Huang Mimi, Chen Xiaohu, Li Yongxin
Key Laboratory of Functional Molecular Solids (Ministry of Education), Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Key Laboratory of Functional Molecular Solids (Ministry of Education), Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Talanta. 2023 May 15;257:124384. doi: 10.1016/j.talanta.2023.124384. Epub 2023 Feb 18.
Solid-nanopores/nanopipettes have the exquisite ability to reveal the changes in molecular volume due to the advantages of adjustable size, good rigidity and low noise. Herein, a new platform for sensing application was established based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes. In this method, GQH was immobilized on gold-coated nanopipette, which could be used as a catalyst for the reaction of HO with 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to promote the conversion of ABTS to ABTS ions inside gold-coated nanopipette, and the change of transmembrane ion current could be monitored in real time. At the optimal conditions, there was a correlation between the ion current and the concentration of HO in a certain range, which could be used for the hydrogen peroxide sensing. The GQH immobilized nanopipette provides a useful platform to investigate enzymatic catalysis in confined environment, which can be used in electrocatalysis, sensing and fundamental electrochemistry.
由于具有尺寸可调、刚性好和噪声低等优点，固体纳米孔/纳米吸管具有揭示分子体积变化的卓越能力。在此，基于G-四链体-血红素DNAzyme（GQH）功能化的金涂层纳米吸管建立了一种用于传感应用的新平台。在该方法中，GQH固定在金涂层纳米吸管上，其可作为HO与2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）反应的催化剂，以促进金涂层纳米吸管内ABTS向ABTS离子的转化，并且可以实时监测跨膜离子电流的变化。在最佳条件下，离子电流与一定范围内的HO浓度之间存在相关性，可用于过氧化氢传感。固定有GQH的纳米吸管为研究受限环境中的酶催化提供了一个有用的平台，可用于电催化、传感和基础电化学。
