Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, P.R. China.
School of Laboratory Medicine, Wannan Medical College, Wuhu, 241000, P.R. China.
Anal Bioanal Chem. 2024 Sep;416(21):4717-4726. doi: 10.1007/s00216-024-05423-5. Epub 2024 Jul 6.
As biomarkers of cancer, the accurate and sensitive detection of microRNAs is of great significance. Therefore, we proposed a surface-enhanced Raman scattering (SERS)/electrochemical (EC) dual-mode nanosensor for sensitively detecting miRNA-141. The nanosensor uses Au@Ag nanowires as a novel SERS/EC sensing platform, which has the advantages of good biocompatibility, fast response, and high sensitivity. The dual-mode nanosensor can not only effectively overcome the problem of insufficient reliability of single signal, but also realize the amplification and stable output of the detection signal, to ensure the reliability and repeatability of miRNA detection. With this sensing strategy, the target miRNA-141 can be detected over a wide linear range (100 fM to 50 nM) (LOD of 18.4 fM for SERS and 16.0 fM for electrochemical methods). In addition, the process shows good selectivity and can distinguish miRNA-141 from other interfering miRNAs. The actual analysis of human serum samples also proves that our strategy has good reliability, repeatability, and has broad application prospects in the field of analysis and detection.
作为癌症的生物标志物，准确、灵敏地检测 microRNA 具有重要意义。因此，我们提出了一种基于表面增强拉曼散射（SERS）/电化学（EC）双模纳米传感器，用于灵敏检测 miRNA-141。该纳米传感器采用 Au@Ag 纳米线作为新型 SERS/EC 传感平台，具有良好的生物相容性、快速响应和高灵敏度等优点。双模纳米传感器不仅可以有效地克服单一信号可靠性不足的问题，还可以实现检测信号的放大和稳定输出，从而确保 miRNA 检测的可靠性和重复性。利用这种传感策略，可以在较宽的线性范围内（100 fM 至 50 nM）检测到靶标 miRNA-141（SERS 的 LOD 为 18.4 fM，电化学方法的 LOD 为 16.0 fM）。此外，该过程表现出良好的选择性，可以区分 miRNA-141 与其他干扰 miRNA。对人血清样本的实际分析也证明了我们的策略具有良好的可靠性、重复性，在分析检测领域具有广阔的应用前景。
