Li Dan, Chen Hui, Gao Xianhui, Mei Xifan, Yang Liqun
Department of Basic Science, Jinzhou Medical University, Jinzhou 121001, China.
Department of Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China.
ACS Sens. 2021 Mar 26;6(3):613-627. doi: 10.1021/acssensors.0c02322. Epub 2021 Mar 4.
Viruses have caused significant damage to the world. Effective detection is required to relieve the impact of viral infections. A biomolecule can be used as a template such as deoxyribonucleic acid (DNA), peptide, or protein, for the growth of silver nanoclusters (AgNCs) and for recognizing a virus. Both the AgNCs and the recognition elements are tunable, which is promising for the analysis of new viruses. Considering that a new virus such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) urgently requires a facile sensing strategy, various virus detection strategies based on AgNCs including fluorescence enhancement, color change, quenching, and recovery are summarized. Particular emphasis is placed on the molecular analysis of viruses using DNA stabilized AgNCs (DNA-AgNCs), which detect the virus's genetic material. The more widespread applications of AgNCs for general virus detection are also discussed. Further development of these technologies may address the challenge for facile detection of SARS-CoV-2.
病毒给世界造成了巨大破坏。需要进行有效检测以减轻病毒感染的影响。生物分子可作为模板，如脱氧核糖核酸（DNA）、肽或蛋白质，用于银纳米簇（AgNCs）的生长以及识别病毒。AgNCs和识别元件均可调谐，这为新型病毒的分析带来了希望。鉴于新型冠状病毒2（SARS-CoV-2）等新病毒迫切需要一种简便的传感策略，本文总结了基于AgNCs的各种病毒检测策略，包括荧光增强、颜色变化、淬灭和恢复。特别强调了使用DNA稳定的AgNCs（DNA-AgNCs）对病毒进行分子分析，其可检测病毒的遗传物质。还讨论了AgNCs在一般病毒检测中更广泛的应用。这些技术的进一步发展可能应对SARS-CoV-2简便检测的挑战。
