Matsumura P, Silverman M, Simon M
J Bacteriol. 1977 Dec;132(3):996-1002. doi: 10.1128/jb.132.3.996-1002.1977.
Hybrid Escherichia coli ColE1 plasmids carrying the genes for motility (mot) and chemotaxis (che) were transferred to a minicell-producing strain. The mot and che genes on the hybrid plasmid directed protein synthesis in minicells. Polypeptides synthesized in minicells were identical to the products of the motA, motB, cheA, cheW, cheM, cheX, cheB, cheY, and cheZ genes previously identified by using hybrid lambda and ultraviolet-irradiated host cells (Silverman and Simon, J. Bacteriol. 130:1317-1325, 1977), thus confirming these gene product assignments. The products of some che genes (cheA and cheM) appeared as more than one band on polyacrylamide gel electrophoresis, but analysis of partial peptide digests of these polypeptides suggested that the multiple forms were coded for by a single gene. Measurement of the physical length of the hybrid plasmids allowed an estimate of the amount of coding capacity of the cloned deoxyribonucleic acid, which was devoted to the synthesis of the mot and che gene products. These estimates were also consistent with the hypothesis that the multiple polypeptides corresponding to cheA and cheM were the products of single genes.
携带运动性基因（mot）和趋化性基因（che）的杂交大肠杆菌ColE1质粒被转移到一个产生微小细胞的菌株中。杂交质粒上的mot和che基因指导微小细胞中的蛋白质合成。在微小细胞中合成的多肽与先前通过使用杂交λ噬菌体和紫外线照射宿主细胞鉴定出的motA、motB、cheA、cheW、cheM、cheX、cheB、cheY和cheZ基因的产物相同（Silverman和Simon，《细菌学杂志》130:1317 - 1325，1977），从而证实了这些基因产物的归属。一些che基因（cheA和cheM）的产物在聚丙烯酰胺凝胶电泳上呈现出不止一条带，但对这些多肽的部分肽段消化分析表明，多种形式是由单个基因编码的。对杂交质粒物理长度的测量使得能够估计克隆的脱氧核糖核酸的编码能力，这些编码能力用于mot和che基因产物的合成。这些估计也与以下假设一致，即对应于cheA和cheM的多种多肽是单个基因的产物。
