Nayar J K, Knight J W, Bradley T J
Florida Medical Entomology Laboratory, IFAS, University of Florida, Vero Beach.
Exp Parasitol. 1988 Jun;66(1):124-31. doi: 10.1016/0014-4894(88)90057-4.
Factors which control the expression of the refractory or susceptible condition to infection with Dirofilaria immitis in the mosquito. Aedes aegypti, were investigated using three protocols. (1) Microfilariae and prelarvae were injected into the hemocoel of susceptible A. aegypti. Some microfilariae and prelarvae developed to the L1 larval stage but they failed to complete development to the infective stage. (2) Enema of microfilariae and prelarvae from infected susceptible and refractory donor females were given into the midgut of uninfected susceptible and refractory recipient females. The results indicate that the conditions which inhibit the initiation of development are present in the Malpighian tubules and not in the midgut of the refractory mosquitoes. (3) Transplants of infected Malpighian tubules from susceptible and refractory donor females were made into the abdominal hemocoel of uninfected susceptible and refractory recipient females. The results showed that the refractory condition depends on the genetic makeup of the donor, not the recipient, mosquito. The above results taken as a whole indicate that the factors which control refractoriness are not present in the midgut but are present in the Malpighian tubule cells of refractory A. aegypti.
利用三种实验方案研究了控制埃及伊蚊对犬恶丝虫感染呈现难治或易感状态表达的因素。（1）将微丝蚴和前期幼虫注入易感埃及伊蚊的血腔。一些微丝蚴和前期幼虫发育到L1幼虫阶段，但未能完成发育至感染阶段。（2）将来自受感染的易感和难治供体雌蚊的微丝蚴和前期幼虫灌肠至未感染的易感和难治受体雌蚊的中肠。结果表明，抑制发育起始的条件存在于马氏管中，而不存在于难治性蚊子的中肠中。（3）将来自易感和难治供体雌蚊的感染马氏管移植到未感染的易感和难治受体雌蚊的腹部血腔。结果表明，难治状态取决于供体而非受体蚊子的基因组成。综合上述结果表明，控制难治性的因素不存在于中肠，而是存在于难治性埃及伊蚊的马氏管细胞中。
