Key Laboratory of Animal Products Processing, Ministry of Agriculture, Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Jiangsu Synergetic Innovation Center of Meat Production and Processing, and College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
Key Laboratory of Animal Products Processing, Ministry of Agriculture, Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Jiangsu Synergetic Innovation Center of Meat Production and Processing, and College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
Food Chem. 2021 Aug 1;352:129376. doi: 10.1016/j.foodchem.2021.129376. Epub 2021 Feb 23.
In the present study, three types of polyphenols, namely, (-)-epigallocatechin-3-gallate (EGCG), catechin (C), and gallic acid (GA), were grafted to myofibrillar protein (MP)-dextran (DX) conjugate through a free radical-mediated adduction method. The analysis of secondary structure showed that conjugation of polyphenols induced a decrease in contents of α-helix structures. The surface hydrophobicity of MP-DX conjugate was increased after polyphenols were covalently adducted, while that of the free amino, thiol groups, and tyrosine residues were decreased, especially with the addition of EGCG (p < 0.05). Analysis of rheological properties showed that covalently linking of polyphenols decreased the thermal gelling capacity by inhibiting myosin-head aggregation and myosin tails interaction. Moreover, polyphenol adduction was able to remarkably improve the thermal stability and antioxidant activity of MP-DX conjugate. The findings regarding enhanced functionalities evidence potential of applying the ternary adduct as a novel antioxidant.
在本研究中，三种类型的多酚，即（-）-表没食子儿茶素-3-没食子酸酯（EGCG）、儿茶素（C）和没食子酸（GA），通过自由基介导的加成方法接枝到肌原纤维蛋白（MP）-葡聚糖（DX）缀合物上。二级结构分析表明，多酚的共轭导致α-螺旋结构含量降低。MP-DX 缀合物的表面疏水性在多酚共价加成后增加，而游离氨基、巯基和酪氨酸残基的表面疏水性降低，特别是添加 EGCG 时（p < 0.05）。流变性能分析表明，通过抑制肌球蛋白头部聚集和肌球蛋白尾部相互作用，多酚的共价连接降低了热胶凝能力。此外，多酚加成能够显著提高 MP-DX 缀合物的热稳定性和抗氧化活性。这些关于增强功能的发现为应用三元加合物作为新型抗氧化剂提供了证据。
