Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy; IIGM Foundation, Italian Institute for Genomic Medicine, Candiolo, Torino, Italy.
IIGM Foundation, Italian Institute for Genomic Medicine, Candiolo, Torino, Italy; Candiolo Cancer Institute, FPO-IRCCS, Candiolo, Torino, Italy.
Trends Cell Biol. 2021 Jul;31(7):542-555. doi: 10.1016/j.tcb.2021.02.002. Epub 2021 Mar 1.
There is increasing evidence that long noncoding RNAs (lncRNAs) are among the main regulatory factors of stem cell maintenance and differentiation. They act through various mechanisms and interactions with proteins, DNA, and RNA. This heterogeneity in function increases the capabilities of the lncRNome toolkit but also makes it difficult to predict the function of novel lncRNAs or even rely on biological information produced in animal models. As lncRNAs are species- and tissue-specific, the recent technical advances in self-renewal and differentiation of human embryonic stem cells (ESCs) make these cells the ideal system to identify key regulatory lncRNAs and study their molecular functions. Here we provide an overview of the functional versatility of lncRNA mechanistic heterogeneity in regulating pluripotency maintenance and human differentiation.
越来越多的证据表明，长非编码 RNA（lncRNA）是干细胞维持和分化的主要调节因子之一。它们通过与蛋白质、DNA 和 RNA 的各种机制和相互作用发挥作用。这种功能的异质性增加了 lncRNome 工具包的功能，但也使得预测新的 lncRNA 的功能变得困难，甚至难以依赖于在动物模型中产生的生物学信息。由于 lncRNA 具有物种和组织特异性，因此人类胚胎干细胞（ESC）自我更新和分化的最新技术进步使这些细胞成为鉴定关键调节 lncRNA 并研究其分子功能的理想系统。在这里，我们概述了 lncRNA 机制异质性在调节多能性维持和人类分化中的功能多样性。
