Jaubert Stéphanie, Laffaire Jean-Baptiste, Abad Pierre, Rosso Marie-Noëlle
INRA, Unité Interactions Plantes-Microorganismes et Santé Végétale, 123 Boulevard Francis Meilland, 60606 Antibes, France.
FEBS Lett. 2002 Jul 3;522(1-3):109-12. doi: 10.1016/s0014-5793(02)02906-x.
The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed.
首个动物多聚半乳糖醛酸酶（PG，EC 2.1.15）编码cDNA，即Mi-pg-1，是从植物寄生线虫南方根结线虫中克隆得到的。在大肠杆菌中进行异源表达后，证实了MI-PG-1的酶活性。MI-PG-1序列上存在预测的信号肽，以及Mi-pg-1基因转录本在感染性幼虫食管腺中的特定定位，这表明MI-PG-1可能被分泌到植物组织中。文中讨论了MI-PG-1在寄生过程中的潜在作用。
