Schönermark S, Filsinger S, Berger B, Hänsch G M
Institut für Immunologie, Universität Heidelberg, FRG.
Immunology. 1988 Apr;63(4):585-90.
C8-binding protein is an intrinsic membrane protein of the human erythrocyte. It inhibits the complement (C5b-9)-mediated lysis in a species-restricting manner. In the present study we incorporated C8bp, isolated from human erythrocytes, into sheep erythrocytes (SRBC). SRBC, normally sensitive to lysis by human C5b-9, became insensitive to lysis. Furthermore, we found that C8bp is incorporated into the membrane-attack complex C5b-9, most probably by interacting with C8, since C8bp has an affinity for C8, particularly for the C8 alpha-gamma-subunit. Antibodies to C8bp react with the C8 alpha-subunits and with C9, pointing to the possibility of a partial homology between these proteins.
C8结合蛋白是人类红细胞的一种内在膜蛋白。它以物种限制的方式抑制补体（C5b-9）介导的细胞溶解。在本研究中，我们将从人类红细胞中分离出的C8结合蛋白（C8bp）整合到绵羊红细胞（SRBC）中。通常对人类C5b-9介导的细胞溶解敏感的SRBC变得对细胞溶解不敏感。此外，我们发现C8bp整合到膜攻击复合物C5b-9中，很可能是通过与C8相互作用，因为C8bp对C8有亲和力，特别是对C8α-γ亚基。针对C8bp的抗体与C8α亚基和C9发生反应，表明这些蛋白之间可能存在部分同源性。
