终末补体蛋白对红细胞溶解的同源物种限制
Homologous species restriction in lysis of erythrocytes by terminal complement proteins.
作者信息
Hänsch G M, Hammer C H, Vanguri P, Shin M L
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5118-21. doi: 10.1073/pnas.78.8.5118.
The cytolytic efficiency of the terminal complement protein complex, C5b-9, varies with the species of origin of C8 and C9. In the present study, we explored the susceptibility of erythrocytes from various species to lysis by C5b6,7 plus C8 and C9 from different species. EC5b6,7 intermediates were prepared on human, guinea pig, rabbit, mouse, and rat erythrocytes with human C5b6 and guinea pig C7. The degree of lysis of these intermediates by C8 and C9 was found to vary widely depending on the species of the proteins and the target cells. In all cases, lysis was least efficient when C8 and C9 were homologous with respect to the target cell species. This effect was mostly attributable to C9. The inefficient lysis in a homologous system is not due to a failure of C9 binding. Rather, the poor lysis in the homologous system may be attributable to inefficient insertion or channel formation.
末端补体蛋白复合物C5b-9的溶细胞效率因C8和C9的来源物种而异。在本研究中，我们探究了来自不同物种的红细胞对由不同物种的C5b6、7加C8和C9所介导的裂解的敏感性。利用人C5b6和豚鼠C7在人、豚鼠、兔、小鼠和大鼠的红细胞上制备了EC5b6,7中间体。发现这些中间体被C8和C9裂解的程度因蛋白质和靶细胞的物种不同而有很大差异。在所有情况下，当C8和C9与靶细胞物种同源时，裂解效率最低。这种效应主要归因于C9。同源系统中裂解效率低下并非由于C9结合失败。相反，同源系统中裂解不佳可能归因于插入效率低下或通道形成。
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