Imaging of Endoplasmic Reticulum Ca in the Intact Pituitary Gland of Transgenic Mice Expressing a Low Affinity Ca Indicator.

The adenohypophysis contains five secretory cell types (somatotrophs, lactotrophs, thyrotrophs, corticotrophs, and gonadotrophs), each secreting a different hormone, and controlled by different hypothalamic releasing hormones (HRHs). Exocytic secretion is regulated by cytosolic Ca signals ([Ca]), which can be generated either by Ca entry through the plasma membrane and/or by Ca release from the endoplasmic reticulum (ER). In addition, Ca entry signals can eventually be amplified by ER release calcium-induced calcium release (CICR). We have investigated the contribution of ER Ca release to the action of physiological agonists in pituitary gland. Changes of [Ca] in the ER ([Ca]) were measured with the genetically encoded low-affinity Ca sensor GAP3 targeted to the ER. We used a transgenic mouse strain that expressed erGAP3 driven by a ubiquitous promoter. Virtually all the pituitary cells were positive for the sensor. In order to mimick the physiological environment, intact pituitary glands or acute slices from the transgenic mouse were used to image [Ca]. [Ca] was measured simultaneously with Rhod-2. Luteinizing hormone-releasing hormone (LHRH) or thyrotropin releasing hormone (TRH), two agonists known to elicit intracellular Ca mobilization, provoked robust decreases of [Ca] and concomitant rises of [Ca]. A smaller fraction of cells responded to thyrotropin releasing hormone (TRH). By contrast, depolarization with high K triggered a rise of [Ca] without a decrease of [Ca], indicating that the calcium-induced calcium-release (CICR) ryanodine receptor amplification mechanism is not present in these cells. Our results show the potential of transgenic ER Ca indicators as novel tools to explore intraorganellar Ca dynamics in pituitary gland .