对结核菌素纯蛋白衍生物迟发型超敏反应的诱导：树突状细胞与5-羟色胺协同作用可替代巨噬细胞。
Induction of delayed-type hypersensitivity responses to PPD: dendritic cells in synergy with 5-hydroxytryptamine can substitute for macrophages.
作者信息
Roberts D, Katz D R, Mukherjee S, Rook G A
机构信息
Bland Sutton Institute of Pathology, University College and Middlesex School of Medicine, London, U.K.
出版信息
Immunology. 1988 Apr;63(4):697-700.
We describe the use of 5-hydroxytryptamine (5HT) as an adjuvant in the induction of the delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). Based upon our previous studies with antigen-pulsed macrophages (M phi), we have shown that both the Day 2 early (2 hr) reaction and the Day 3 (24 hr) reaction are augmented if 5HT is incorporated into the priming injection. Furthermore, we have confirmed that in contrast to M phi, antigen-pulsed dendritic cells (DC) fail to prime the early (2 hr) component of DTH. However, DC do prime the early response if injected along with 5HT. A peripheral 5HT antagonist, ICS 205-930, inhibits both the M phi-mediated and the 5HT/DC-primed reactions. These findings support the hypothesis that DTH reactions require a cascade of both inflammatory and immunological signals, and that in mice vascular permeability mediated via 5HT is important in the early phase of the reaction.
我们描述了使用5-羟色胺（5HT）作为佐剂来诱导对纯化蛋白衍生物（PPD）的迟发型超敏反应（DTH）。基于我们之前对抗原脉冲巨噬细胞（M phi）的研究，我们发现，如果将5HT加入初次注射中，第2天的早期（2小时）反应和第3天（24小时）反应都会增强。此外，我们已经证实，与M phi不同，抗原脉冲树突状细胞（DC）不能引发DTH的早期（2小时）成分。然而，如果与5HT一起注射，DC确实能引发早期反应。外周5HT拮抗剂ICS 205-930可抑制M phi介导的反应以及5HT/DC引发的反应。这些发现支持了这样的假设，即DTH反应需要一系列炎症和免疫信号，并且在小鼠中，经由5HT介导的血管通透性在反应的早期阶段很重要。
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