Durbeck Julia, Breton Celine, Suter Michael, Luth Eric S, McGehee Annette M
Biology Department, Suffolk University, Boston, MA, USA.
Department of Biology, Simmons University, Boston, MA, USA.
PeerJ. 2021 Feb 25;9:e10892. doi: 10.7717/peerj.10892. eCollection 2021.
The Doublesex/Mab-3 Domain transcription factor DMD-10 is expressed in several cell types in , including in the nervous system. We sought to investigate whether DMD-10 is required for normal neuronal function using behavioral assays. We found that mutation of did not broadly affect behavior. mutants were normal in several behavioral assays including a body bends assay for locomotion, egg laying, chemotaxis and response to gentle touch to the body. mutants did have defects in nose-touch responsiveness, which requires the glutamate receptor GLR-1. However, using quantitative fluorescence microscopy to measure levels of a GLR-1::GFP fusion protein in the ventral nerve cord, we found no evidence supporting a difference in the number of GLR-1 synapses or in the amount of GLR-1 present in mutants. mutants did have decreased responsiveness to high osmolarity, which, along with nose-touch, is sensed by the polymodal sensory neuron ASH. Furthermore, mutation of impaired behavioral response to optogenetic activation of ASH, suggesting that promotes neuronal signaling in ASH downstream of sensory receptor activation. Together our results suggest that DMD-10 is important in regulating the frequency of multiple ASH-dependent behavioral responses.
双性/ Mab - 3结构域转录因子DMD - 10在多种细胞类型中表达，包括在神经系统中。我们试图通过行为分析来研究DMD - 10对于正常神经元功能是否是必需的。我们发现[基因名称]的突变并没有广泛地影响行为。[突变体名称]突变体在几种行为分析中表现正常，包括用于运动的身体弯曲分析、产卵、趋化作用以及对身体轻柔触摸的反应。[突变体名称]突变体在鼻触反应性方面确实存在缺陷，而这需要谷氨酸受体GLR - 1。然而，使用定量荧光显微镜来测量腹侧神经索中GLR - 1::GFP融合蛋白的水平，我们没有发现证据支持[突变体名称]突变体中GLR - 1突触数量或GLR - 1含量存在差异。[突变体名称]突变体对高渗透压的反应性确实降低了，高渗透压与鼻触一样，是由多模态感觉神经元ASH感知的。此外，[基因名称]的突变损害了对ASH光遗传学激活的行为反应，这表明[基因名称]在感觉受体激活下游促进ASH中的神经元信号传导。我们的结果共同表明，DMD - 10在调节多种依赖ASH的行为反应频率方面很重要。
