Alsayegh Shaikha Y, Al-Ghouti Mohammad A, Zouari Nabil
Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University. PO. Box 2713, Doha, Qatar.
Biotechnol Rep (Amst). 2021 Feb 10;29:e00598. doi: 10.1016/j.btre.2021.e00598. eCollection 2021 Mar.
To enhance the process of bacterial remediation of weathered hydrocarbons, the area of Dukhan, Qatar, was considered as a model for weathering processes. Self-purification by indigenous hydrocarbon-degrading bacteria showed low performance. Biostimulation/seeding using one or another of the indigenous bacteria improved the performance. Symbiosis between three strains dominating the soil; D11, D12, and D13, was highly performant for removal of total petroleum hydrocarbons in the weathered soil. D11, the most sensitive, showed the highest performance when mixed with D12 or D13. D12, less performant than D11, was more active on diesel range organics (DRO: C10-C28), similar to D11. D13 showed a metabolic behavior close to commensal and co-metabolic ones. It was more active on hydrocarbons above C29. Combination of the three strains conducted to the removal of at least 80% of C10-C35 organics in the extract at concentrations of 31.1 mg/g TPH-DRO.
为了加强对风化烃类的细菌修复过程，卡塔尔的杜汉地区被视为风化过程的一个模型。本地烃降解细菌的自我净化表现不佳。使用一种或另一种本地细菌进行生物刺激/接种提高了性能。在土壤中占主导地位的三株菌株D11、D12和D13之间的共生关系，对于去除风化土壤中的总石油烃具有很高的性能。最敏感的D11与D12或D13混合时表现出最高的性能。性能不如D11的D12对柴油范围有机物（DRO：C10 - C28）的活性更高，与D11相似。D13表现出接近共生和共代谢的代谢行为。它对C29以上的烃类更具活性。这三株菌株的组合在浓度为31.1 mg/g TPH - DRO的提取物中能去除至少80%的C10 - C35有机物。
