Mahanta Sudarshana, Khanikor Bulbuli
Department of Zoology, Gauhati University, Guwahati, 781014, India.
Heliyon. 2021 Feb 17;7(2):e06128. doi: 10.1016/j.heliyon.2021.e06128. eCollection 2021 Feb.
Mosquito control programme using synthetic insecticides has been facing the challenges of resistance development. However, synergistic combinations of plant essential oils (EOs) having different modes of actions and potent lethal toxicity may further negate the concern of resistance development.
In this study, the toxicity of 28 EOs and the two synthetic insecticides, Temephos and Malathion were evaluated individually and based on the performance, binary combinations of effective EOs were prepared and tested against the larval and adult stages of . Mixtures were prepared by blending LC10 or LD10 concentration/doses of candidates at different volume ratios.
Results demonstrated that among 155 numbers of combinations of different volume ratios, 1:1 ratio of (bulbs) L.+ (peels) Macfd. (AsB + CpP) was found to be the most potent against adults, whereas, 1:1 volume ratio of (bulbs)+ (leaves) (AsB + CpL) was found to possess highest activity against larvae after considering its dose and synergistic interaction. GC-MS analysis revealed the presence of diallyl trisulfide, diallyl disulfide, beta-citronellol, ocimene as major constituents of AsB + CpP combination and diallyl disulfide, linalool, citronellal, caryophyllene oxide etc. in AsB + CpL combination.
Therefore, the said mixtures of the plant essential oils and or mixtures of the constituent compounds can be used as effective control agents for the control of the filarial vector, .
使用合成杀虫剂的蚊虫控制计划一直面临着抗药性发展的挑战。然而，具有不同作用方式和强大致死毒性的植物精油（EOs）的协同组合可能会进一步消除对抗药性发展的担忧。
在本研究中，分别评估了28种植物精油以及两种合成杀虫剂（双硫磷和马拉硫磷）的毒性，并根据性能制备了有效的植物精油二元组合，并针对其幼虫和成虫阶段进行了测试。通过将候选物的LC10或LD10浓度/剂量以不同体积比混合来制备混合物。
结果表明，在155种不同体积比的组合中，发现（鳞茎）L. +（果皮）Macfd.（AsB + CpP）的1:1比例对成虫最有效，而在考虑其剂量和协同相互作用后，发现（鳞茎）+（叶子）（AsB + CpL）的1:1体积比对幼虫具有最高活性。气相色谱-质谱分析表明，AsB + CpP组合的主要成分是二烯丙基三硫化物、二烯丙基二硫化物、β-香茅醇、罗勒烯，而AsB + CpL组合中含有二烯丙基二硫化物、芳樟醇、香茅醛、氧化石竹烯等。
因此，所述植物精油混合物和/或组成化合物的混合物可作为控制丝虫病媒介的有效控制剂。
